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Skin type
Cardiothoracic Surgery
Drug modeling
Endoscopic class
Open abdominal class
Imaging services
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Cell platform
Biochemical testing platform
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antibody
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Functional laboratory
Animal platform
Cell platform
Biochemical testing platform
Pathology detection platform
Molecular and protein platforms
Behavioral platform
Instrument display (renamed)
Rat and rat treadmills
Ultrasound detection of cardiac function in rats and mice
Non-invasive blood pressure monitor
Live imaging of small animals
MRI detection
X-ray testing
ECG collection
Muscle tension detection
Your Current Position:
Functional laboratory
细胞增殖检测
2024-04-16
本公司应用MTT、CCK-8比色法,检测细胞存活和生长。
细胞瞬时/稳定转染
2024-04-16
细胞转染的基本原理是将外源的DNA克隆到载体上后导入细胞,借助宿主细胞表达载体上的目的片段,从而使目的基因在细胞中发挥功能。目前转染方法有多种,如脂质体转染法、磷酸钙-DNA共沉淀法、电穿孔转染法、腺病毒感染等。
原代细胞分离培养与鉴定
2024-04-16
原代细胞是来源于不同组织器官,胚胎及血液的新鲜样本经特殊实验方法处理而制备的原初培养细胞,这一过程被称为原代细胞分离。细胞的分离纯化和细胞培养技术是细胞生物实验的基础。
载体构建
2024-04-15
载体构建(vectorconstruction),是把目的DNA分子运送到受体细胞中去,因此必须寻找一种能进入细胞、装载了外来的DNA片段后仍能照样复制的运载体。
定点突变
2024-04-15
定点突变是指通过聚合酶链式反应(PCR)等方法向目的DNA片段(可以是基因组,也可以是质粒)中引入所需变化(通常是表征有利方向的变化),包括碱基的添加、删除、点突变等。
甲基化检测
2024-04-15
DNA甲基化在DNA甲基化转移酶(DNMTs)的作用下的CpG二核苷酸5’端的胞嘧啶转变为5’-甲基胞嘧啶,是最早发现的修饰途径之一。
SNP
2024-04-15
SNP,即单核苷酸多态性(SingleNucleotide Polymorphism,SNP),主要是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性。与其它分子标记相比,SNP 分辨率最高也最为丰富,覆盖基因组范围大,遗传上比较稳定。
siRNA
2024-04-15
siRNA是一种小RNA分子(~21-25核苷酸),由Dicer(RNAase Ⅲ家族中对双链RNA具有特异性的酶)加工而成。
miRNA
2024-04-15
小 RNA是 19~28 nt 的调控RNA分子,主要包括微 RNA(micro RNA,miRNA)和小干涉RNA(short interfering RNA,siRNA)两类。
实时荧光定量PCR
2024-04-15
实时荧光定量PCR技术,是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测每次聚合酶链式反应(PCR)循环后产物总量的方法,最后通过标准曲线(内参)对未知模板(待测样品)进行定量分析的方法。
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