Respiratory disease model

Respiratory disease model


Disease model

Modeling   approach

Animal strains

Measurements

Guinea pig model of asthma

Intraperitoneal injection of 10%OVA   sensitization +OVA continuous atomization excitation

Hartleyguinea pig

Breathing pattern analysis;  Total cells and eosinophils counts were performed in BALF; Histological assessment of lung

Chronic bronchitis rat model

Passive   smoking

SD/Wistar rat

Histological assessment of lungAnalysis of inflammatory cell infiltration   in bronchial wall and base; Analysis of alveolar septal lesions

Chronic obstructive pulmonary disease rat   model

(COPD-rat)

Repeatedly instilled with LPS intratracheally on the first day and on the 14th   day; On days 2–13 days and 15–28 days, the rats were placed in a self-produced   fumigating box for smoking

SD/Wistar rat

 the forced expiratory volume in 0.3   s/forced vital capacity (FEV0.3/FVC%) was lower and inspiratory   resistance (RI) was higher in COPD model; Histological assessment of lung

Pneumonia rat model

Tail vein injection of 10mg/kg LPS

Wistar   rat

Lung injury, macroscopic observation of   large area bleeding exudation; Histological assessment of lung; Detection of   inflammatory factors

Pulmonary emphysema mouse model

intratracheal instillation of pancreatic   porcine elastase

C57BL/6   mice

fractional area of alveolar collapse, and   hyperinflated alveoli increased; Elastic fiber content decreased; Collagen fiber content increased; Lung inflammation

Pulmonary fibrosis rat model

Bleomycin was intratrachally aspirated

SD/Wistar rat

Histological assessment of lung; Inflammatory cells count in BALF; Hydroxyproline detection

guinea pig model of allergic rhinitis(AR)

Intranasally ovalbumin sensitization

Hartley guinea pig

Sneezing and nose rubbing; Nasal blockade, leukocyte infiltration, and   lung inflation pressure

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Case analysis    Pulmonary fibrosis rat model

Idiopathic  pulmonary fibrosis (IPF) is a chronic, diffuse, progressive disease of the pulmonary interstitium with agnogenic etiology, and it lacks effective therapy in the clinic now. Now, experimental research of IPF commonly induces the model by intratracheal injection of bleomycin (BLM) in rodents, It has been observed in the experimental model induced by BLM that the histopathologic changes of lung tissue were mainly inflammation of alveoli in early stage and then gradual progression of fibrosis deposition.


Experimental animal information

Name   of animal model

Pulmonary fibrosis rat model

Species and strains of animal

SD/   Wistar rat

Gender of animal

Age of animal

adult

Weight of animal

180~220g


Caracteristics

Control group revealed normal lung tissue structure and slight collagen deposition in the alveolar septa. Treatment with BLM revealed severe collagen deposition, obliteration of interalveolar spaces, and disordered lung structure. Alveolar space on day 28 was almost occluded by fibrosis and normal alveoli were observed scarcely. Hydroxyproline in BLM group were significantly higher when compared to that of control group. Total cell count increased in the BALF of BLM treated rats compared with that in the BALF of control rats, The total number of macrophages and neutrophils significantly increased in BLM treated rats.

Partial result display


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Masson’s trichrome (MT) stain(×200


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Determination of hydroxyproline content