In recent years, the incidence rate, disability rate and even fatality rate of limb ischemia-reperfusion injury caused by skeletal muscle ischemia-reperfusion have been increasing year by year. If no timely and effective treatment is carried out, the degree of damage to patients will be great, and even remote organ damage will be caused in severe cases. The current methods for constructing models of limb ischemia-reperfusion injury mainly involve general non-invasive vascular clamping or non-invasive suture ligation of the femoral artery for ischemia-reperfusion operations, as well as methods for inducing limb ischemia-reperfusion injury by external closure of femoral artery blood flow.

Observation indicators

The paws of rats turn pale, bluish purple, and cool, indicating that the blood flow of the femoral artery has been successfully blocked. If the paws of rats change from dark purple to pink, it indicates the restoration of blood flow perfusion; Pathological examination of femoral artery and gastrocnemius muscle, etc.

Partial experimental diagram display